Composite

Part:BBa_K3826007:Design

Designed by: Lucas Müller   Group: iGEM21_Duesseldorf   (2021-10-21)


promoter pRha mVenus activator rhaS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 132
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1291
    Illegal BamHI site found at 758
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 156
    Illegal AgeI site found at 279
    Illegal AgeI site found at 1023
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct is only functional with the activator rhaS and is necessary when the system is established in other organisms.


Source

This Composite part contains three different parts. The promoter pRha and the activator rhaS can be found in E.coli. The yellow fluorescent protein mVenus which was published in 2006 can be found in Aequorea victoria.

References